|
KMID : 0380420190430040239
|
|
Journal of Prventive Veterinary Medicine
2019 Volume.43 No. 4 p.239 ~ p.241
|
|
|
High-yield production of porcine parvovirus VP2 protein of Korean strain assembled into virus-like particles in a baculovirus expression system
|
|
Park Se-Yeon
Lee Ju-Yeon
Song Jae-Young
Cho Soo-Dong
Lee Jienny
Hyun Bang-Hun
Ouh In-Ohk
|
|
|
Abstract
|
|
|
|
Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.
|
|
|
KEYWORD
|
|
|
Expression, porcine parvovirus, PPV, purification, VP2 protein
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|